Correlation between serum macrophage migration inhibitory factor and regulated on activation normal T cell expressed and secreted cytokines in patients with chronic hepatitis B / 中国医师进修杂志

Objective To investigate the serum levels and correlation between macrophage migration inhibitory factor (MIF) and regulated on activation normal T cell expressed and secreted cytokines (RANTES) in patients with chronic hepatitis B (CHB).Methods Forty-four CHB patients (CHB group)and 30 healthy controls (control group) were enrolled in this study.The venous blood was collected and serum MIF and RANTES levels were detected by enzyme-linked immunosorbent assay.Correlation between MIF and RANTES was analyzed in CHB group.Results The serum MIF and RANTES levels in CHB group were significantly higher than those in control group [(8.48 ± 1.70) μ g/L vs.(1.99 ± 2.38) μ g/L,(3.94 ±2.38) μ g/L vs.(0.33 ± 0.15) μ g/L,P =0.000].There was no correlation between MIF level and RANTES level(r =0.212,P＞ 0.05).Conclusions The serum MIF and RANTES levels are significantly increased in patients with CHB,but there is no correlation.The participation pathogenesis way of CHB is different.

Rhinovirus-Infected Epithelial Cells Produce More IL-8 and RANTES Compared With Other Respiratory Viruses

PURPOSE: The environmental factors human rhinoviruses (HRVs) and house dust mites (HDMs) are the most common causes of acute exacerbations of asthma. The aim of this study was to compare the chemokine production induced by HRVs in airway epithelial cells with that induced by other respiratory viruses, and to investigate synergistic interactions between HRVs and HDMs on the induction of inflammatory chemokines in vitro. METHODS: A549 human airway epithelial cells were infected with either rhinovirus serotype 7, respiratory syncytial virus (RSV)-A2 strain, or adenovirus serotype 3 and analyzed for interleukin (IL)-8 and regulated on activation, normal T-cell expressed and secreted (RANTES) release and mRNA expression. Additionally, activation of nuclear factor (NF)-kappaB and activator protein (AP)-1 were evaluated. The release of IL-8 and RANTES was also measured in cells stimulated simultaneously with a virus and the HDM allergen, Der f1. RESULTS: HRV caused greater IL-8 and RANTES release and mRNA expression compared with either RSV or adenovirus. NF-kappaB and AP-1 were activated in these processes. Cells incubated with a virus and Der f1 showed an increased IL-8 release. However, compared with cells incubated with virus alone as the stimulator, only HRV with Der f1 showed a statistically significant increase. CONCLUSIONS: IL-8 and RANTES were induced to a greater extent by HRV compared with other viruses, and only HRV with Der f1 acted synergistically to induce bronchial epithelial IL-8 release. These findings may correspond with the fact that rhinoviruses are identified more frequently than other viruses in cases of acute exacerbation of asthma.

Serum ECP, RANTES and Eotaxin Levels in Infants with Bronchiolitis / 소아과

PURPOSE: Viral respiratory infection, especially respiratory syncytial(RS) virus, can predispose sufferers to the development of asthma by mechanisms that are presently undetermined. The role of inflammatory mediators in pathogenesis of asthma and RS virus disease is not well-understood. We investigated the performance of serum eosinophil cationic protein(ECP), regulated on activation normal T-cell expressed and secreted(RANTES) and eotaxin levels on the airway inflammation in a population of patients with RS virus and non-RS virus bronchiolitis, compared with control subjects. METHODS: Twenty-eight patients with RS virus positive bronchiolits, 11 patients with RS virus negative bronchiolitis and seven control subjects were enrolled in the study. Serum ECP, RANTES and eotaxin levels were measured by enzyme immunoassay. We analyzed the correlation among serum ECP, RANTES, eotaxin, immunoglobulin E and blood eosinophil count in the RS virus positive bronchiolitis group and the RS virus negative bronchiolitis group, respectively. RESULTS: Both serum ECP and RANTES in the brochiolitis group were not higher than in the control. Serum eotaxin concentration in bronchiolitis group was significantly higher than in control(P= 0.030), but there was no significant difference between RS virus positive and negative groups. There were significant correlations between the peripheral blood eosinophil count and serum ECP, and between the levels of serum ECP and RANTES in the RS virus positive bronchiolitis group(P=0.038, 0.012 respectively). CONCLUSION: Serum eotaxin may have a systemic effect in pathophysiology of bronchiolitis but ECP and RANTES may not. However, they may have some indirect effects inferred from the correlations among them.